Comparison of tacrolimus concentrations measured by the IMx tacrolimus II vs the PRO-TRAC II FK506 ELISA assays.
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چکیده
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منابع مشابه
Analytical validation of the PRO-Trac II ELISA for the determination of tacrolimus (FK506) in whole blood.
BACKGROUND The analytical validation of multiple lots of the PRO-Trac II ELISA (DiaSorin) for the determination of tacrolimus in whole blood is described. METHODS The analytical parameters assessed included analytical sensitivity, dilution linearity, functional sensitivity, values in samples containing no tacrolimus, intra- and interassay precision, supplementation and recovery, metabolite cr...
متن کاملClinical efficacy of the Abbott Tacrolimus II assay for the IMx.
Monitoring tacrolimus is essential to maintain therapeutic concentrations. Performance of the new Abbott Tacrolimus assay (FK II) was evaluated and compared to the original tacrolimus assay (FK I). 189 trough whole blood samples from transplant cases were included in the study. Samples (n = 117) with FK I concentrations > 5 ng/mL were reanalyzed with the FK II assay. Patient samples (n = 43) th...
متن کاملCYP3A5 genotype does not influence the blood concentration of tacrolimus measured with the Abbott immunoassay.
To the Editor: Therapeutic drug monitoring of immunosuppressants is well established as an aid to optimizing patient management after allograft transplantation. Most laboratories measure cyclosporin and tacrolimus by immunoassays. However, measured values may be affected by the presence of circulating metabolites because of the limited specificity of the antibodies used in these assays. Cyclosp...
متن کاملComparison of the Abbott IMx Tacrolimus I and Tacrolimus II assays.
References 1. Chomczynski P, Sacchi N. Single step method of RNA isolation by acid guanidinidium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156–9. 2. Sambrook J, Fritsch EF, Maniatis, T. Molecular cloning, a laboratory manual, 2nd ed. Plainview, NY: Cold Spring Harbor Laboratory Press, 1989:7.28–7.52. 3. Tominaga K, Miura Y, Arakawa T, Kobayashi K, Mitsuhashi M. Colorimetri...
متن کاملMulti-site analytical evaluation of the Abbott ARCHITECT tacrolimus assay.
The objective of this study was to evaluate the analytical performance of the Abbott ARCHITECT Tacrolimus immunoassay. Proficiency panels and specimens from a population of organ transplant recipients were analyzed in 6 clinical laboratories in Europe and the United States, and the results were compared with other methods. The ARCHITECT assay requires a whole blood specimen pretreatment step wi...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 45 10 شماره
صفحات -
تاریخ انتشار 1999